Mimivirus giant particles incorporate a large fraction of 1 anonymous and unique gene products 2 3 Running Title : Mimivirus proteome 4 5
نویسندگان
چکیده
5 Patricia Renesto, Chantal Abergel, Philippe Decloquement, Danielle Moinier, Saïd 6 Azza, Hiroyuki Ogata, Patrick Fourquet, Jean-Pierre Gorvel, Jean-Michel Claverie 7 and Didier Raoult 8 9 10 From the Unité des Rickettsies, CNRS UMR 6020, IFR-48, Faculté de Médecine, 27 Boulevard 11 Jean Moulin, the Information Génomique & Structurale, CNRS UPR 2589, IBSM, Parc 12 scientifique et technologique de Luminy, 163 Avenue de Luminy, case 934, the Institut de 13 Biologie Structurale et Microbiologie, 31 Chemin Joseph Aiguier, the Centre d'Immunologie 14
منابع مشابه
Mimivirus giant particles incorporate a large fraction of anonymous and unique gene products.
Acanthamoeba polyphaga mimivirus is the largest known virus in both particle size and genome complexity. Its 1.2-Mb genome encodes 911 proteins, among which only 298 have predicted functions. The composition of purified isolated virions was analyzed by using a combined electrophoresis/mass spectrometry approach allowing the identification of 114 proteins. Besides the expected major structural c...
متن کاملAdvances in Mimivirus Pathogenicity
Viral diseases in the clinical setting have been extensively investigated. Viruses are now considered as potentially responsible for nosocomial infections, especially in intensive care unit (ICU) patients. Mimivirus is the largest virus known to date. Recent studies have suggested that Mimivirus could be responsible for both community-acquired and nosocomial pneumonia. These studies were mainly...
متن کاملMimivirus-like Particles in Acanthamoebae from Sewage Sludge
al. Burden of disease caused by Strepto-coccus pneumoniae in children younger than 5 years: global estimates. al. Dried blood spot real-time polymerase chain reaction assays to screen newborns for congenital cytomegalovirus infection.rial load in children and adolescents with pneumococcal pneumonia and empyema. cation of pneumococcal serotypes from culture-negative clinical specimens by novel r...
متن کاملIdentification of giant Mimivirus protein functions using RNA interference
Genomic analysis of giant viruses, such as Mimivirus, has revealed that more than half of the putative genes have no known functions (ORFans). We knocked down Mimivirus genes using short interfering RNA as a proof of concept to determine the functions of giant virus ORFans. As fibers are easy to observe, we targeted a gene encoding a protein absent in a Mimivirus mutant devoid of fibers as well...
متن کاملUltrastructural Characterization of the Giant Volcano-like Virus Factory of Acanthamoeba polyphaga Mimivirus
Acanthamoeba polyphaga Mimivirus is a giant double-stranded DNA virus defining a new genus, the Mimiviridae, among the Nucleo-Cytoplasmic Large DNA Viruses (NCLDV). We used utrastructural studies to shed light on the different steps of the Mimivirus replication cycle: entry via phagocytosis, release of viral DNA into the cell cytoplasm through fusion of viral and vacuolar membranes, and finally...
متن کامل